Evaluation of a fluorescent approach to implement monoclonality assurance using calcein-AM viability dye

Carola Mancini

Assessing monoclonality is key to the establishment of a cell line, and evidence of monoclonality is required by regulatory agencies to get a biopharmaceutical drug to the marketplace.

In this study, we demonstrate an optimized workflow using the fluorescence reagent, calcein-AM (CAM), in conjunction with a fluorescence-capable CloneSelect™ Imager (CSI-FL) that shows similar viability to label-free conditions while simultaneously providing high assurance of clonality.

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